(A) Effects of AICAR, gAd, and creatine on p70 S6 Kinase phosphorylation in L6 myotubes. L6 cells were incubated with 1 mM AICAR for 1 hour, 2.5 μg/ml gAd for 2 hours, and 10mM creatine for 2 hours (0.5 mM creatine preincubation for 2 days), and then p70 S6 kinase phosphorylation at Thr389 were measured by western blot, and p70 S6K was used as a control; P-p70 S6K; phosphorylated p70 S6 Kinase. Con: control; Cr: creatine; AIC: AICAR; gAd: globular adiponectin. (B) Quantification of data of AICAR, gAd, and creatine on p70 S6 Kinase phosphorylation in L6 myotubes. ** P < 0.01 v. control.
