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Figure 7: Effect of AICAR, gAd, and creatine on p70 S6 kinase phosphorylation in L6 myotubes. (A) Effects of AICAR, gAd, and creatine on p70 S6 Kinase phosphorylation in L6 myotubes. L6 cells were incubated with 1 mM AICAR for 1 hour, 2.5 μg/ml gAd for 2 hours, and 10mM creatine for 2 hours (0.5 mM creatine preincubation for 2 days), and then p70 S6 kinase phosphorylation at Thr389 were measured by western blot, and p70 S6K was used as a control; P-p70 S6K; phosphorylated p70 S6 Kinase. Con: control; Cr: creatine; AIC: AICAR; gAd: globular adiponectin. (B) Quantification of data of AICAR, gAd, and creatine on p70 S6 Kinase phosphorylation in L6 myotubes. ** P < 0.01 v. control. |