Figure 8: Role of adenylate kinase in the action of gAd
(A) Adenylate kinase 1 (AK1) protein expression treated with AK1 siRNA in L6 myotubes. L6 cells were incubated with or without 100 nM AK1 siRNA for 3 days and then protein expression was measured by western blot described under “Materials and methods”. β-actin was used as a control; Con: control; AK1: AK1 siRNA. (B) Effect of AK1 siRNA on gAd induced glucose uptake in L6 myotubes. After transfected by AK1 siRNA, L6 cells were incubated with 5 mM glucose with or without 2.5 μg/ml gAd for 2 hours, and then glucose uptake was measured as described under “Materials and methods”.