(A) LKB1 protein expression treated with LKB1 siRNA in L6 myotubes. L6 cells were incubated with or without 100 nM LKB1 siRNA for 3 days and then protein expression was measured by western blot described under “Materials and methods”. ConsiRNA was used as a control; Con: control; ConsiRNA: control siRNA (non-targeting siRNA). (B) Effect of LKB1 siRNA on gAd induced glucose uptake in L6 myotubes. After LKB1 siRNA transfection, L6 cells were incubated with 5 mM glucose with or without 2.5 μg/ml of gAd for 2 hours, and then glucose uptake was measured as described under “Materials and methods”. * p < 0.05 versus control (Con); ** p < 0.01 versus gAd.
