Figure 12: Effect of creatine on protein expression of phosphofructokinase 1 (PFK 1) in L6 myotubes
(A) PFK1 protein expression treated with creatine. L6 cells were preincubated with or without 0.5 mM creatine for 2 days. After 2 h serum free, L6 cells were treated with or without 10 mM creatine for 2 h, and then protein expression of phosphofructokinase 1 (PFK 1) was measured by western blot as described under “Materials and methods”. β-actin was used as a control. Con: control; Cr: creatine. (B) (D) Quantification of data for 12 (A). Quantification studies were performed using the Image Analysis Tools of Fluor ChemTM (Alpha Innotech Corpor-ation, CA). * P < 0.05 v. control.