| Name |
Location |
Size (bp) |
Sequence |
| |
446–600 |
154 |
5’-TCCTCCGGCCCCTCAATGCG-3’ |
| pEV-R |
|
|
5’-ATTGTCACCATAAGCAGCCA-3’ |
| pEV71-F |
2372–2392 |
226 |
5’-GCAGCCCAAAAGAACTTCAC-3’ |
| pEV71-R |
2578–2598 |
|
5’-ATTTCAGCAGCTTGGAGTGC-3’ |
| pCA16-F |
2335–2355 |
208 |
5’-ATTGGTGCTCCCACTACAGC-3’ |
| pCA16-R |
2523–2543 |
|
5’-TCAGTGTTGGCAGCTGTAGG-3’ |
| pEV71-VP1-F |
2367–2282 |
1015 |
5’-CGGGATCCGGAGATAGGGTGGCAGATGTA-3’ |
| pEV71-VP1-R |
|
|
5’-CGGAATCCAGTAGTGATCGCCGTGCG-3’ |
Primer-EV-forward coupled with primer-EV-reverse were designed as universal primers of enterovirus, and used to amplify a 154-bp segment shared by enteroviruses,
including EV71, EV70, coxsackie virus A, and poliovirus. Primer-EV71-forward and -reverse were designed to detect EV71, while pCA16-forward and -reverse were for
CoxA16 detection. Primer EV71-VP1-forward and -reverse were used to construct pET28a-VP1. |
