Detection Dilution fold
VP1 1:1 1:2 1:4 1:8 1:16 1:32 1:64
Positive serum A A 1:128 1:128 1:64 1:32 1:16
Different antigen concentrations were used to sensitize 1% latex beads with an overnight reaction time (as the starting condition recommended by the manufacturer). Standard positive serum was serially diluted for reaction with the sensitized latex beads.
Table 2: Optimization of protein concentration for latex bead sensitization