Sensitization time 2 3 4 5 6 7 8 S T LAT S T LAT S T LAT S T LAT S T LAT S T LAT S T LAT 1:1 + 1:1 ++ 1:1 +++ 1:1 ++++ 1:1 ++++ 1:1 ++++ 1:1 ++++ 1:2 +/- 1:2 ++ 1:2 ++ 1:2 +++ 1:2 ++++ 1:2 ++++ 1:2 ++++ 1:4 - 1:4 + 1:4 ++ 1:4 +++ 1:4 +++ 1:4 +++ 1:4 +++ 1:8 - 1:8 - 1:8 ++ 1:8 ++ 1:8 +++ 1:8 +++ 1:8 +++ 1:16 - 1:16 - 1:16 + 1:16 ++ 1:16 +++ 1:16 +++ 1:16 +++ 1:32 - 1:32 - 1:32 - 1:32 ++ 1:32 ++ 1:32 ++ 1:32 ++ 1:64 - 1:64 - 1:64 - 1:64 + 1:64 ++ 1:64 ++ 1:64 ++ 1:128 - 1:128 - 1:128 - 1:128 - 1:128 + 1:128 + 1:128 + 1:256 - 1:256 - 1:256 - 1:256 - 1:256 - 1:256 - 1:256 - VP1 proteins at a 1:8 dilution were used to sensitize 1% latex beads with various reaction times (2, 3, 4, 5, and 6 hours). The best reaction time was determined via a reaction with standard positive serum in serial twofold dilutions (from 1:1 to 1:256).

Table 3: Optimization of reaction time for latex bead sensitization