Glucose Xylose Minimum ethanol production 0.00 0.00 Maximum ethanol production 18.49 15.71 Maximum Predicted yield (% of theoretical maximum) 92.45 94.08 biomass 0.10 0.07 Targets Pyruvate formate lyase Pyruvate formate lyase G6P isomerase G6P isomerase

Table 1: Results of GDLS simulations. Uptake rates for both glucose and xylose were chosen as 10 mmol/gDW/h. Product secretion rates are in mmol/gDW·hr. Ethanol yield is shown in percentage of maximum yield.