WT PFK, SGL, PFL PFK, SGL, PFL, LDH PFK, SGL, PFL, LDH, FRD PFL, LDH, FRD Biomass 0.28 0.16 0.16 0.16 0.16 Glucose uptake rate 16.52 15.00 15.00 15.00 15.00 Ethanol 12.38 8.47 14.63 17.47 15.89 Lactate 1.31 9.23 0.00 0.00 0.00 Formate 22.44 0.00 0.00 0.00 0.00 Acetate 10.30 5.25 5.52 5.30 4.55 Succinate 0.80 5.55 8.05 0.05 0.05 minimum ethanol flux* 0 0 13.42 13.56 12.82

* FBA prediction Abbreviations: PFK: Phosphofructokinase; SGL: Sedoheptulose 1,7-Bisphosphate D-Glyceraldehyde-3-Phosphate-Lyase; FBA: Fructose-Bisphosphate Aldolase; PFL: Pyruvate Formate Lyase; FRD: Fumarate Reductase.

Table 3: Hybrid MOMA-predicted fluxes through major products. All flux values except shown in mmol/gDW·hr. Biomass flux shown in hr-1. The minimum ethanol flux was calculated using FBA by minimizing the flux through this reaction and not through hybrid MOMA.