Drug concentration (μg) % of viability   % of cell death  
  24h 48h 24h 48h
0.078 88.664 ± 0.01 87.38 ± 0.02 11.34 ± 0.02 12.62 ± 0.01
0.156 48.36 ± 0.02 39.9 ± 0.02 51.64 ± 0.02 60.1 ± 0.01
0.312 17.784 ± 0.01 14.03 ± 0.01 82.21 ± 0.01 85.7 ± 0.02
0.625 8.684 ± 0.01 9.303 ± 0.01 91.31 ± 0.02 90.97 ± 0.02
1.25 1.352 ± 0.01 1.143 ± 0.02 98.64 ± 0.01 98.87 ± 0.01
2.5 0.416 ± 0.02 0.467 ± 0.01 99.58 ± 0.03 99.53 ± 0.03
5 1.456 ± 0.03 1.61 ± 0.03 98.54 ± 0.02 98.39 ± 0.02
Vehicle control 97.8 ± 0.02 97.5 ± 0.02 2.2 ± 0.0 2.5 ± 0.0
Doxorubicin 10.13 ± 0.1 07.0 ± 0.1 89.87 ± 0.2 93 ± 0.1
IC50 0.156 μg
Table 3: In vitro apoptotic activity of endophytic coumarin from Alternaria species on HeLa cancer cell lines.