| Key |
Metabolites |
δ 1H (ppm) and multiplicity* |
Concentration (µM/mg) |
Estimates of absolute concentrations in tissue (mM) |
| |
|
|
|
| |
|
|
Mean ± SD |
Mean ± SD |
| |
|
|
Control |
3 Gy |
7.8 Gy |
Control |
3 Gy |
7.8 Gy |
| 1 |
Leucine |
0.94 (t), 0.99 (d), 1.27 (m), 1.46 (m), 1.95 (m), 3.64 (d) |
1.83 ± 0.30 |
2.78 ± 0.22 |
4.44 ± 1.02 |
0.96 ± 0.16 |
1.46 ± 0.12 |
2.34 ±0.54 |
| 2 |
2-Aminobutyrate |
0.96 (t), 1.91 (m), 3.68 (t) |
1.48 ± 0.06 |
2.23 ± 0.20 |
2.37 ± 0.45 |
0.78 ± 0.03 |
1.17 ± 0.11 |
1.25 ± 0.24 |
| 3 |
Valine |
0.97 (d), 1.02 (d), 2.27 (m), 3.6 (d) |
1.37 ± 0.17 |
2.38 ± 0.20 |
4.21 ± 0.71 |
0.72 ± 0.09 |
1.25 ± 0.11 |
2.21 ± 0.37 |
| 4 |
Isobutyrate |
1.05 (d), 2.38 (m) |
0.22 ± 0.04 |
0.17 ± 0.01 |
0.22 ± 0.02 |
0.12 ± 0.02 |
0.09 ± 0.01 |
0.12 ± 0.01 |
| 5 |
3-Hydroxyisobutyrate |
1.08 (d), 2.48 (m), 3.53 (m), 3.69 (m) |
0.27 ± 0.05 |
0.19 ± 0.02 |
0.31 ± 0.05 |
0.14 ± 0.03 |
0.10 ± 0.01 |
0.16 ± 0.03 |
| 6 |
3-Hydroxybutyrate |
1.2 (d), 2.3 (dd), 2.39 (dd), 4.14 (dd) |
2.60 ± 0.32 |
2.66 ± 0.32 |
1.41 ± 0.11 |
1.37 ± 0.17 |
1.40 ± 0.17 |
0.74 ± 0.06 |
| 7 |
Fucose |
1.2 (m), 3.4 (dd), 3.63 (dd), 3.8 (m), 4 (m), 4.19 (m), 4.55 (d), 5.22 (m) |
1.20 ± 0.17 |
1.46 ± 0.24 |
1.68 ± 0.43 |
0.63 ± 0.09 |
0.77 ± 0.13 |
0.88 ± 0.23 |
| 8 |
Threonine |
1.33 (d), 3.58 (d), 4.26 (m) |
4.69 ± 0.51 |
7.05 ± 0.91 |
6.97 ± 0.97 |
2.47 ± 0.27 |
3.71 ± 0.48 |
3.67 ± 0.51 |
| 9 |
Lactate |
1.33 (d), 4.11(q) |
10.89 ± 1.24 |
18.7 ± 1.97 |
24.51 ± 1.57 |
5.73 ± 0.65 |
9.84 ± 1.04 |
12.9 ± 0.83 |
| 10 |
Lysine |
1.43 (m), 1.51 (m), 1.72 (m), 1.89 (m), 1.91 (m), 3.03 (t),3.75 (t), |
1.13 ± 0.20 |
1.54 ± 0.08 |
1.61 ± 0.19 |
0.59 ± 0.10 |
0.81 ± 0.04 |
0.85 ± 0.10 |
| 11 |
Alanine |
1.48 (d), 3.78 (q) |
3.55 ± 0.52 |
4.66 ± 0.76 |
7.06 ± 1.15 |
1.87 ± 0.27 |
2.45 ± 0.4 |
3.71 ± 0.61 |
| 12 |
Arginine |
1.65 (m), 1.74 (m), 1.9 (m), 1.92 (m), 3.23 (t), 3.77 (t) |
2.04 ± 0.21 |
3.04 ± 0.40 |
3.58 ± 0.28 |
1.07 ± 0.11 |
1.60 ± 0.21 |
1.88 ± 0.15 |
| 13 |
γ-Glutamylphenylalanine |
1.97 (m),2.34 (m),2.89 (dd),3.21 (dd),3.57 (t),4.46 (m),7.27 (t),7.35 (t),7.91 (d) |
0.23 ± 0.02 |
0.25 ± 0.03 |
0.33 ± 0.03 |
0.12 ± 0.01 |
0.13 ± 0.01 |
0.17 ± 0.01 |
| 14 |
Glutamate |
2.05 (m), 2.14 (m), 2.34 (m), 2.37 (m), 3.76 (dd) |
21.34 ± 3.37 |
21.65 ± 1.30 |
18.44 ± 1.52 |
11.23 ± 1.77 |
11.4 ± 0.69 |
9.71 ± 0.80 |
| 15 |
Glutathione |
2.16 (m), 2.18 (m), 2.52 (m), 2.57 (m), 2.95 (dd), 2.98 (dd), 3.74 (d), 3.77 (m), 4.58 (m) |
1.92 ± 0.15 |
2.65 ± 0.36 |
3.27 ± 0.54 |
1.01 ± 0.08 |
1.40 ± 0.19 |
1.72 ± 0.29 |
| 16 |
Malate |
2.36 (dd), 2.67 (dd), 4.3 (m) |
2.18 ± 0.14 |
2.79 ± 0.27 |
2.67 ± 0.44 |
1.14 ± 0.07 |
1.47 ± 0.14 |
1.40 ± 0.23 |
| 17 |
Succinate |
2.39 (s) |
1.13 ± 0.28 |
1.10 ± 0.09 |
0.91 ± 0.22 |
0.60 ± 0.15 |
0.58 ± 0.05 |
0.48 ± 0.12 |
| 18 |
2-Oxoglutarate |
2.44 (t), 2.99 (t) |
1.53 ± 0.11 |
2.06 ± 0.24 |
2.83 ± 0.38 |
0.81 ± 0.06 |
1.08 ± 0.12 |
1.49 ± 0.20 |
| 19 |
Isocitrate |
2.49 (m), 2.57 (m), 2.99 (m), 4.06 (d) |
3.02 ± 0.53 |
2.83 ± 0.25 |
3.98 ± 0.38 |
1.59 ± 0.28 |
1.49 ± 0.13 |
2.10 ± 0.20 |
| 20 |
β-Alanine |
2.53 (t), 3.20 (t) |
1.73 ± 0.18 |
2.23 ± 0.61 |
2.41 ± 0.13 |
0.91 ± 0.09 |
1.17 ± 0.32 |
1.27 ± 0.07 |
| 21 |
Citrate |
2.54 (d), 2.68 (d) |
0.19 ± 0.08 |
0.28 ± 0.04 |
0.28 ± 0.04 |
0.10 ± 0.04 |
0.15 ± 0.02 |
0.15 ± 0.02 |
| 22 |
Aspartate |
2.67 (dd), 2.82 (dd), 3.9 (dd) |
10.22 ± 1.49 |
10.77 ± 1.17 |
11.16 ± 1.25 |
5.38 ± 0.78 |
5.67 ± 0.61 |
5.87 ± 0.66 |
| 23 |
Trimethylamine |
2.88 (s) |
0.15 ± 0.03 |
0.15 ± 0.02 |
0.15 ± 0.01 |
0.08 ± 0.02 |
0.08 ± 0.01 |
0.08 ± 0.01 |
| 24 |
Tyramine |
2.92 (t), 3.23 (t), 6.9 (m), 7.2 (m) |
0.05 ± 0.01 |
0.07 ± 0.01 |
0.20 ± 0.06 |
0.02 ± 0.01 |
0.03 ± 0 |
0.11 ± 0.03 |
| 25 |
Creatine phosphate |
3.01 (s), 3.94 (s) |
0.57 ± 0.11 |
0.61 ± 0.07 |
0.84 ± 0.06 |
0.30 ± 0.06 |
0.32 ± 0.04 |
0.44 ± 0.03 |
| 26 |
Creatine |
3.04 (s), 3.91 (s) |
0.76 ± 0.12 |
1.67 ± 0.17 |
1.81 ± 0.15 |
0.40 ± 0.06 |
0.88 ± 0.09 |
0.95 ± 0.08 |
| 27 |
Creatinine |
3.04 (s), 3.98 (s) |
0.63 ± 0.11 |
0.64 ± 0.05 |
1.25 ± 0.08 |
0.33 ± 0.06 |
0.34 ± 0.03 |
0.66 ± 0.04 |
| 28 |
Tyrosine |
3.06 (dd), 3.2 (dd), 3.93 (dd), 6.9 (m), 7.2 (m) |
0.35 ± 0.04 |
0.65 ± 0.11 |
1.04 ± 0.10 |
0.18 ± 0.02 |
0.34 ± 0.06 |
0.54 ± 0.05 |
| 29 |
Phenylalanine |
3.11 (dd), 3.27 (dd), 3.99 (dd), 7.33 (m), 7.38 (m), 7.43 (m) |
0.41 ± 0.08 |
0.74 ± 0.11 |
1.36 ± 0.33 |
0.22 ± 0.04 |
0.39 ± 0.06 |
0.72 ± 0.18 |
| 30 |
Histidine |
3.14 (dd), 3.24 (dd), 3.98 (dd), 7.06 (s), 7.79 (s) |
0.21 ± 0.05 |
0.17 ± 0.04 |
0.27 ± 0.02 |
0.11 ± 0.02 |
0.09 ± 0.02 |
0.14 ± 0.01 |
| 31 |
Ethanolamine |
3.14 (m), 3.82 (m) |
2.54 ± 0.41 |
2.31 ± 0.42 |
2.65 ± 0.58 |
1.34 ± 0.21 |
1.21 ± 0.22 |
1.39 ± 0.31 |
| 32 |
Choline |
3.21 (s), 3.52 (m), 4.07 (m) |
2.18 ± 0.39 |
1.35 ± 0.19 |
2.43 ± 0.27 |
1.15 ± 0.21 |
0.71 ± 0.10 |
1.28 ± 0.14 |
| 33 |
π-Methylhistidine |
3.22 (dd), 3.3 (dd), 3.73 (s), 3.95 (dd), 6.8 (s), 8 (s) |
0.18 ± 0.02 |
0.29 ± 0.08 |
0.42 ± 0.09 |
0.09 ± 0.01 |
0.15 ± 0.04 |
0.22 ± 0.05 |
| 34 |
O-Phosphoethanolamine |
3.22 (m), 3.98 (m) |
18.22 ± 1.18 |
15.93 ± 1.33 |
13.69 ± 1.38 |
9.59 ± 0.62 |
8.39 ± 0.70 |
7.21 ± 0.73 |
| 35 |
Glucose |
3.23 (m), 3.4 (m), 3.5 (m), 3.53 (dd), 3.7 (dd), 3.72 (dd),3.78 (m), 3.83 (m), 3.84 (m), 3.94 (dd), 4.65 (d), 5.23 (d) |
1.49 ± 0.31 |
1.49 ± 0.32 |
2.84 ± 0.80 |
0.78 ± 0.17 |
0.79 ± 0.17 |
1.50 ± 0.42 |
| 36 |
Trimethylamine N-oxide |
3.23 (s) |
0.95 ± 0.15 |
0.74 ± 0.17 |
0.98 ± 0.15 |
0.50 ± 0.08 |
0.39 ± 0.09 |
0.51 ± 0.08 |
| 37 |
sn-Glycero-3-phosphocholine |
3.23 (s), 3.6 (dd), 3.67 (m), 3.68 (dd), 3.86 (m), 3.92 (m),3.95 (m), 4.32 (m) |
5.49 ± 0.73 |
7.41 ± 0.98 |
7.71 ± 1.38 |
2.89 ± 0.38 |
3.90 ± 0.52 |
4.06 ± 0.72 |
| 38 |
Taurine |
3.26 (t), 3.43 (t) |
39.14 ± 1.12 |
44.86 ± 1.26 |
53.7 ± 4.46 |
20.6 ± 0.59 |
23.61 ± 0.66 |
28.26 ± 2.35 |
| 39 |
Betaine |
3.27 (s), 3.91 (s) |
1.71 ± 0.38 |
1.04 ± 0.37 |
1.07 ± 0.22 |
0.90 ± 0.20 |
0.55 ± 0.19 |
0.56 ± 0.12 |
| 40 |
myo-Inositol |
3.28 (t), 3.53 (dd), 3.62 (t), 4.06 (m) |
7.03 ± 0.69 |
12.3 ± 1.39 |
18.98 ± 3.24 |
3.70 ± 0.36 |
6.48 ± 0.73 |
9.99 ± 1.70 |
| 41 |
Tryptophan |
3.3 (dd), 3.48 (dd), 4.05 (dd), 7.2 (dd), 7.29 (dd), 7.32 (s),7.54 (d), 7.74 (d), |
0.16 ± 0.03 |
0.28 ± 0.02 |
0.33 ± 0.06 |
0.08 ± 0.02 |
0.15 ± 0.01 |
0.17 ± 0.03 |
| 42 |
UDP-glucose |
3.51 (dd), 3.58 (m), 3.78 (dd), 4.13 (d), 4.17 (m), 4.23 (m), 4.27 (m), 4.36 (m), 5.61 (dd), 5.97 (d), 5.99 (d),7.97 (d) |
1.08 ± 0.12 |
0.86 ± 0.01 |
0.78 ± 0.09 |
0.57 ± 0.06 |
0.45 ± 0.01 |
0.41 ± 0.05 |
| 43 |
UDP-glucuronate |
3.51 (dd), 3.58 (m), 3.78 (dd), 4.13 (d),4.17 (m),4.23 (m), 4.27 (m), 4.36 (m), 5.61 (dd), 5.97 (d), 5.99 (d),7.97 (d) |
0.34 ± 0.02 |
0.29 ± 0.06 |
0.26 ± 0.03 |
0.18 ± 0.01 |
0.15 ± 0.03 |
0.14 ± 0.02 |
| 44 |
Glycerol |
3.56 (dd), 3.65 (dd), 3.78 (m) |
1.81 ± 0.18 |
2.58 ± 0.23 |
3.52 ± 0.41 |
0.95 ± 0.10 |
1.36 ± 0.12 |
1.85 ± 0.21 |
| 45 |
Glycine |
3.56 (s) |
3.76 ± 0.85 |
5.99 ± 0.88 |
4.25 ± 0.97 |
1.98 ± 0.45 |
3.16 ± 0.46 |
2.23 ± 0.51 |
| 46 |
UDP-galactose |
3.72 (dd), 3.75 (dd), 3.81 (m), 3.91 (dd), 4 (d), 4.16 (m),4.19 (m), 4.24 (m), 4.28 (m), 4.37 (m), 5.63 (dd), 5.98 (m), 7.95 (d) |
0.27 ± 0.04 |
0.29 ± 0.06 |
0.29 ± 0.03 |
0.14 ± 0.02 |
0.15 ± 0.03 |
0.15 ± 0.02 |
| 47 |
Ascorbate |
3.73 (m),4.00 (t),4.50 (d) |
9.00 ± 1.13 |
7.98 ± 0.71 |
6.32 ± 2.49 |
4.74 ± 0.59 |
4.20 ± 0.38 |
3.33 ± 1.31 |
| 48 |
Uridine |
3.8 (dd), 3.89 (dd), 4.13 (m), 4.23 (t), 4.36 (t), 5.9 (d),5.92 (d), 7.88 (d), |
0.57 ± 0.17 |
0.70 ± 0.11 |
0.56 ± 0.05 |
0.30 ± 0.09 |
0.37 ± 0.06 |
0.29 ± 0.02 |
| 49 |
Cytidine |
3.81 (dd), 3.92 (dd), 4.12 (m), 4.19 (t), 4.3 (m), 5.89 (d),6.06 (d), 7.81(d) |
0.33 ± 0.07 |
0.26 ± 0.03 |
0.26 ± 0.04 |
0.17 ± 0.04 |
0.14 ± 0.02 |
0.14 ± 0.02 |
| 50 |
Adenosine |
3.83 (dd), 3.91 (dd), 4.29 (m), 4.42 (dd), 4.79 (dd), 6.05 (d), 8.24 (s), 8.34 (s) |
0.75 ± 0.18 |
0.67 ± 0.15 |
0.42 ± 0.07 |
0.39 ± 0.09 |
0.36 ± 0.08 |
0.22 ± 0.04 |
| 51 |
Inosine |
3.84 (dd), 3.92 (dd), 4.27 (m), 4.44 (m), 4.76 (t), 6.11 (d),8.23 (s), 8.35 (s) |
0.71 ± 0.39 |
0.27 ± 0.08 |
0.31 ± 0.21 |
0.37 ± 0.20 |
0.14 ± 0.04 |
0.16 ± 0.11 |
| 52 |
Serine |
3.84 (dd), 3.95 (dd), 3.99 (dd) |
6.75 ± 1.23 |
7.21 ± 0.65 |
8.96 ± 0.41 |
3.55 ± 0.65 |
3.80 ± 0.34 |
4.72 ± 0.22 |
| 53 |
AMP |
4.01 (m), 4.36 (m), 4.49 (m), 4.78 (dd), 6.11 (d), 8.24 (s),8.62 (s) |
1.30 ± 0.41 |
1.16 ± 0.17 |
1.07 ± 0.16 |
0.69 ± 0.22 |
0.61 ± 0.09 |
0.56 ± 0.08 |
| 54 |
ADP |
4.2 (m), 4.37 (m), 4.57 (dd), 4.74 (dd), 6.13 (dd), 8.27 (s),8.58 (s) |
0.69 ± 0.08 |
0.37 ± 0.02 |
0.41 ± 0.05 |
0.36 ± 0.04 |
0.19 ± 0.01 |
0.22 ± 0.03 |
| 55 |
ATP |
4.2 (m), 4.4 (m), 4.57 (dd), 4.74 (dd), 6.15 (d), 8.23 (s),8.38 (s) |
0.39 ± 0.18 |
0.24 ± 0.04 |
0.56 ± 0.07 |
0.21 ± 0.10 |
0.13 ± 0.02 |
0.29 ± 0.04 |
| 56 |
GTP |
4.24 (m), 4.35 (m), 4.55 (m), 4.74 (dd), 5.94 (d), 8.12 (s) |
0.64 ± 0.11 |
0.61 ± 0.15 |
0.41 ± 0.06 |
0.34 ± 0.06 |
0.32 ± 0.08 |
0.22 ± 0.03 |
| 57 |
Uracil |
5.8 (d), 7.53 (d) |
0.06 ± 0.01 |
0.14 ± 0.02 |
0.22 ± 0.06 |
0.03 ± 0.01 |
0.07 ± 0.01 |
0.11 ± 0.03 |
| 58 |
Fumarate |
6.52 (s) |
0.08 ± 0.02 |
0.08 ± 0.02 |
0.08 ± 0.02 |
0.04 ± 0.01 |
0.04 ± 0.01 |
0.04 ± 0.01 |
| 59 |
Niacinamide |
7.6 (dd), 8.26 (m), 8.71 (m), 8.94 (d) |
0.31 ± 0.09 |
0.34 ± 0.04 |
0.42 ± 0.05 |
0.16 ± 0.05 |
0.18 ± 0.02 |
0.22 ± 0.03 |
| 60 |
Oxypurinol |
8.19 (s) |
19.09 ± 2.98 |
10.28 ± 1.49 |
20.43 ± 0.90 |
10.05 ± 1.57 |
5.41 ± 0.78 |
10.75 ± 0.47 |
| 61 |
Hypoxanthine |
8.19 (s), 8.21 (s) |
0.50 ± 0.07 |
0.59 ± 0.20 |
0.68 ± 0.06 |
0.26 ± 0.04 |
0.31 ± 0.10 |
0.36 ± 0.03 |
|
