|
Control |
ONM |
OHG |
OTD |
TLR4 antibody |
without |
with |
without |
with |
without |
with |
without |
with |
pretreatment |
TLR4 mRNA/18s |
1.03 ± 0.14 |
1.07 ± 0.13 |
1.43 ± 0.19a |
1.26 ± 0.14a* |
2.20 ± 0.25ab |
2.62 ± 0.20ab* |
2.15 ± 0.23abc |
1.44 ± 0.19 abc* |
TLR4 Protein /β-actin |
0.16 ± 0.05 |
0.15 ± 0.03 |
0.28 ± 0.06a |
0.17 ± 0.05a* |
0.40 ± 0.12ab |
0.38 ± 0.12ab* |
0.85 ± 0.08abc |
0.19 ± 0.05 abc* |
NF-κB p65 protein/β-actin |
0.16 ± 0.02 |
0.13 ± 0.03 |
0.26 ± 0.06a |
0.14 ± 0.03 a* |
0.44 ± 0.05ab |
0.19 ± 0.04ab* |
0.86 ± 0.07abc |
0.21 ± 0.08abc* |
TNF-α(pg/ml) |
225.79 ± 24.29 |
226.82 ± 26.72 |
253.30 ± 31.57a |
224.43 ± 26.71a* |
336.34 ± 26.55ab |
316.97 ± 35.75ab* |
456.79 ± 19.68abc |
412.87 ± 30.24abc* |
IL-1β(pg/ml) |
135.96 ± 12.73 |
133.19 ± 16.15 |
253.30 ± 31.57a |
132.78 ± 23.67a* |
197.94 ± 16.13 |
183.51 ± 19.30ab* |
268.74 ± 14.50abc |
243.05 ± 19.72abc* |
|
ap < 0.05 as compared with controls under the same pretreatment ; bp < 0.05 as compared with ONM under the same pretreatment; cp < 0.05 as compared with OHG under
the same pretreatment; *p < 0.05 as compared with the same group after 48 h treatment. |
Table 2: TLR4, NF-κB phosphorylation levels, TNF-α and IL-1β levels induced by the serum from obese in THP-1 cells preincubated with or without TLR4 monoclonal
antibody. ONM: Obesity with Normal Metabolism; OHG: Obesity with Hyperglycemia; OTD: Obesity with Three Kinds of Metabolic Disorders. |