Figure 5: Kinetics of accumulation of plant HSPs in TYLCV R and S infected tomatoes. a. Immunodetection of cytoplasmic, chloroplastic HSP70 and BiP in leaf tissue after 7, 14, 21, 28, 35, 42, 56 dpi. HSPs were detected using anti-HSP70 cytosolic, chloroplastic and ER antibodies. Patterns of plant protein OE33 were used as internal control. b. Relative transcript levels of BiP's transcription factor bZIP34, normalized to the host genes. Values were obtained by real-time quantitative RTPCR in leaves of tomatoes, infected during prolonged time.