Figure 3: MicroRNA targeting metabolic enzymes affect SCC cell survival upon cisplatin exposure. (A). SCC-11M cells were transfected with the scrambled (Scr) RNA or siRNA against SREBF2, ATOX1 or G6PD for 48-72hours. Immunoblotting was performed with indicated antibodies. β-actin was used as a loading control. (B). Cell viability assay. SCC-11M cells were transfected with the scrambled (Scr) RNA, indicated microRNA mimics and siRNAs for 36hours and subsequently exposed to control media (CON) or to 10 μg/ml cisplatin (CIS) for 16hours. Cell viability assay was performed in triplicate (*, p<0.05).