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Figure 9: Modulation of metabolic reactions by specific microRNA in SCC-11M cells. (A). Left Panel: CPS1 metabolic reaction. Right Panel: Quantification of Citrulline (CIT). SCC-11 cells and SCC-11M cells were transfected with the scrambled microRNA, miR-146b-3p and siRNA against CPS1 for 36hours, and subsequently exposed to control medium (CON) or 10 μg/ml cisplatin (CIS) for an additional 16h. (B). Left Panel: FADS1 metabolic reaction. Right Panel: Quantification ofEicosapentaenoate (EPA) and Arachidonate (AA). SCC-11 cells and SCC-11M cells were transfected with the scrambled microRNA, miR-203a and siRNA against FADS1 for 32h, and subsequently exposed to control medium (CON) or 10 μg/ml cisplatin (CIS) for an additional 16h. (C). Left Panel: Quantification of Phosphoetanolamine (PE). ETNK1 metabolic reaction. Right Panel: SCC-11 cells and SCC-11M cells were transfected with the scrambled microRNA, miR-382-3p and siRNA against ETNK1 for 36h, and subsequently exposed to control medium (CON) or 10μg/ml cisplatin (CIS) for an additional 16hours. Data presented as relative ratios towards SCC-11 cells grown in control (CON) conditions (designated as 1). Experiments were performed in triplicate in three independent settings (p ≤ 0.05). OTC, ornithine carbamoyltransferase; HUFA, highly unsaturated fatty acids; DHGLA, dihomo-gamma-linolenic acid; ETA, eicosatetraenoicacid. |