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Figure 2: Dual luciferase reporter assay quantitation of the effects of anti- or pre- miR-22 or miR-23a interaction with the 3′ UTR of AQP9. The plasmid constructs together with anti- or pre- miR-22 or miR-23a (30 nM) were co-transfected independently into HepG2 cells. Relative luminescence for luciferase gene activity in treated samples were obtained 48 hours posttransfection by normalizing the values against control transfected with corresponding anti or pre scrambled miRNAs. Data are presented as mean ± SEM (n=3) against control. Statistically significant differences are tested at *p< 0.05 significance. |