Figure 4: Altered expression of miR-22, miR-23a and AQP9 in the livers of diabetic rat models. In comparison to the corresponding controls, AQP9 was observed to be increased in liver samples of diabetic rats while both miR-22 and miR-23a showed significant down-regulation. Data was normalized against the housekeeping gene 18S RNA and presented as mean ± SEM (rats study n=12, 6 normal rats and 6 high fat diet+streptozotocin treated rats). AQP9 protein expression in the livers of normal and high fat diet+streptozotocin treated (Diabetic) rats is also shown (see insert). β-actin was used as loading control. The data presented here is a representative of six independent experiments