Figure 5: Relative glycerol-dependent glucose production in HepG2 cells. HepG2 cells were transfected with anti or pre miR-22 or miR-23a (30 nM) for a period of 48hour. Post-transfection cells were supplemented with 0.5mM glycerol as the primary gluconeogenic substrate and incubated for another 8 hours for glucose production to take place. At the end of 8 hours incubation, medium was collected in tubes for measurement of glucose productions (glucose assay). Medium collected from untreated cells without glycerol was used as blank. Data presented as mean ± SEM (n=3) of glucose output in μg/ ml against control cells transfected with corresponding anti or pre scrambled miRNAs. Statistically significant differences are tested at p< 0.05 significance. *p< 0.05, **p< 0.01.