Sr. No.

Parameter

Bergenin

(+)-Catechin

Gallicin

Gallic acid

b-Sitosterol

1

Instrumental precision

(% CV, n = 7)

3.29

3.36

3.05

1.20

0.85

2

Repeatability

(% CV, n = 5)

1.95

1.83

1.15

1.12

1.51

4

Accuracy

(average % recovery)

100.38

101.45

102.38

99.9

99.92

5

Limit of detection (ng)

60

60

48

64

20

6

Limit of quantification

(ng)

160

160

112

112

60

7

Specificity

Specific

Specific

Specific

Specific

Specific

8

Linearity

(Correlation coefficient)

0.999

0.997

0.998

0.996

0.995

9

Range (ng/ spot)

160-720

160-720

160-720

160-720

80-480

Table 2: Method validation parameters for the quantification of Bergenin, (+)-Catechin, Gallicin, Gallic acid and -Sitosterol by the proposed TLC densitometric methods.