Marker

Amount of marker present (µg)

Amount of marker added (µg)

Amount of marker found (µg)

Recovery* (%)

Average Recovery (%)

Bergenin

110

55

160.25 ± 3.50

97.12 ± 0.73

100.38

110

110

222.5 ± 7.23

101.13 ± 1.18

110

140

255.23 ± 4.71

102.9 ± 1.02

(+)-Catechin

60

30

92.37 ± 4.58

102.63 ± 0.96

101.45

60

60

118.91 ± 7.25

99.09 ± 1.20

60

75

138.59 ± 2.17

102.65 ± 0.64

Gallicin

40

20

60.89 ± 1.21

101.48 ± 0.52

102.38

40

40

83.05 ± 4.15

103.81 ± 0.87

40

50

91.68 ± 3.17

101.86 ± 0.69

Gallic acid

252

125

376.54 ± 3.21

99.87 ± 0.73

99.9

252

252

504.72 ± 2.31

100.14 ± 0.58

252

316

566.25 ± 3.37

99.69 ± 0.76

β-Sitosterol

45

22

67.54 ± 4.71

100.81 ± 1.05

99.92

45

        45

89.75 ± 7.14

99.72 ± 1.19

45

        54

98.23 ± 3.13

99.22 ± 0.87


*Mean ± SD (n=3)
Table 4: Recovery studies of Bergenin, (+)-Catechin, Gallicin, Gallic acid and -Sitosterol at 50 %, 100 % and 125 % addition by the proposed TLC densitometric method.