Figure 2: Chromatogram of amiodarone hydrochloride and its impurities (D and E), 0.01 mg mL-1 of each. Mobile phase: buffer pH 5.0, methanol, acetonitrile (30:30:40, v/v/v), flow rate 1.0 mL min-1, injection volume 10 ÁL. Column: C18 (5 Ám, 150 mm length, 4.6 mm inner diameter), UV detection: 240 nm. Peaks identifi cation: (1) amiodarone impurity D (2): amiodarone impurity E (3): amiodarone hydrochloride.