| (a) By HPTCL |
Parameters |
Levocetirizine |
Montelukast |
Linearity range |
500-2500
ng spot-1 |
1000-5000 ng spot-1 |
r2 ± S.D. |
0.9981 ± 0.0007 |
0.9982 ± 0.002 |
Slope ± S.D. |
2.768 ± 0.005 |
1.756 ± 0.001 |
Intercept ± S.D |
961 ± 1.92 |
3602 ± 3.40 |
Confidence limit of slopea |
2.547-2.990 |
1.618-1.893 |
Confidence limit of intercepta |
594.2-1328
|
3146-4057 |
Sy .X |
109.9 |
136.5 |
|
| (b) By HPCL |
Parameters |
Levocetirizine |
Montelukast |
Linearity range |
1 – 10 µg mL-1 |
2 – 20 µg mL-1 |
r2 ± S.D. |
0.9987 ± 0.00 |
0.998 ± 0.01 |
Slope ± S.D. |
40810 ± 19.44 |
56040 ± 21.61 |
Intercept ± S.D |
-4930 ± 8.04 |
15460 ±6.36 |
Intercept ± S.D |
960.4 ± 1.92 |
3601 ± 3.40 |
| Confidence limit of slopea |
39120 - 42510 |
1.618 -1.893 |
Confidence limit of intercepta |
-14250 - 4389
|
3146 - 4057 |
| Sy .X |
5126 |
136.5 |
*p<0.0001 - Slope significantly different from zero
a95% confidence limit.
Sy.X- Standard deviation of residuals from line. |
| Table 1: Linear regression data for calibration curves. |
