Parameters

SD of peak area*

% RSD

LEVO.

MONT.

LEVO.

MONT.

Mobile phase composition (± 0.1 ml of ammonia)

2.78

3.12

0.75

1.12

Amount of mobile phase (±5 %)

1.54

2.36

0.17

0.32

Time from spotting to chromatography (10 min)

2.53

2.61

0.42

0.54

Time from chromatography to scanning (10 min)

1.74

2.42

0.14

0.33

Factor

  Level

    Retention time

      Asymmetry

LEVO.

MONT.

LEVO.

MONT.

A: Flow Rate (mL min^-1)

0.9

-1

3.643

7.49

1.24

1.06

1.0

0

3.558

7.45

1.23

1.03

1.1

+1

3.462

7.41

1.19

1.04

Mean ± S.D (n =3)

3.56± 0.095

7.45 ±

0.040

1.21± 0.032

1.04 ±

0.036

B: % of methanol in the mobile phase (v/v)

24

-1

3.629

7.59

1.23

1.06

25

0

3.558

7.45

1.13

1.03

26

+1

3.487

7.31

1.17

1.04

Mean ± S.D (n =3)

3.558± 0.071

7.55±

0.14

1.17± 0.05

1.04± 0.02