Figure 1: Typical chromatogram of pharmaceutical combination containinig 0.05 mg/ml of betamethasone, and 0.5 mg/ml of clotrimazole. Column: C18 (25cm X 4.0mm i.d). Mobile phase: methanol/buffer/acetonitrile (33:27:40, v/v). Flow rate: 1.5ml/min; λ: 254 nm. Peaks identification, B: Betamethasone, C: Clotrimazole, a, b: Excipients from the cream preparation.