Parameter Betahistine
dihydrochloride
Etilefrine
hydrochloride
Reaction Temperature(°C)
Reaction Time (min)
          Reagent Volume
(ml of 0.1% w/v in methanol)
Solvent
Maximum Wavelength
90
30

1.5
Water
496
90
30

1.5
Water
503
Table 1: Assay parameters for the determination of betahistine dihydrochloride and etilefrine hydrochloride using fixed time method.