Figure 3: CCTα siRNA sequences induce cell death cell lines lacking p53. H1299 (lung carcinoma cells that lack expression of p53 protein) cultured to 80% confluence were transfected with siRNA S and CCTα siRNAs A, B and C (0–400 nM), preincubated with FuGENE 6 at room temperature as indicated above. Cells were processed for imaging, CTB and LDH assays as indicated above. Light microscopy (Figure not shown), CTB assay (Figure 3A) and LDH assay (Figure 3B) demonstrated that CCTα siRNA induced death in cells lacking p53. On the contrary cells that were treated with siRNA S did not show significant changes in intensity fluorescence and absorbencies.