Parameters Observation
Linearity range ( ng per band) 300 - 1800
Limit of detection (ng) 17.21
Limit of quantitation (ng) 52.18
% Recovery ( n =  9)  100.50
% RSD 0.37
Precision (% RSD)  
Repeatability ( n = 6) (standard) 0.88
Intra- day (n = 6) )(standard) 0.06
Inter-day (n = 6) )(standard) 0.37
Ruggedness  (%RSD)  
Analyst –I (n = 6) )(standard) 0.76
Analyst –II (n = 6) (standard) 0.75
Robustness Robust
Specificity Specific
Table 4: Summary of validation parameters.