Parameters Chemometric methods    
  CLS PCR PLS
Range(μg/ml) 8-12 μg/ml 8-12 μg/ml 8-12 μg/ml
Slop 0.9917 0.9921 0.9929
S.E. of slop 0.0498 0.0490 0.0491
Intercept 0.1129 0.0987 0.0966
S.E. of Intercept 0.5038 0.4999 0.4968
r 0.9992 0.9994 0.9998
LOD 0.45 0.44 0.45
LOQ 1.37 1.35 1.36
Precision 1.13-1.21 1.13-1.21 1.02-1.01
Table 3: (Assay validation obtained by applying the proposed chemometric methods).