Method     2D   2DD   Sample No. %Deg. St.conc (μg/ml) calc.conc (μg/ml) R% calc.conc (μg/ml) R% 1 10% 9 9.09 101.00 8.79 97.67 2 20% 8 8.01 100.13 7.84 98.00 3 30% 7 6.89 98.43 6.93 99.00 4 40% 6 6.05 100.83 5.95 99.17 5 50% 5 5.09 101.80 5.04 100.80 6 60% 4 3.99 99.75 3.99 99.75 7 70% 3 3.06 102.00 2.93 97.67 8 80% 2 2.05 102.50 1.80* 90.36* Mean±SD       100.80±1.339   98.87±1.172 RSD%       1.328   1.173 * Not included in calculations.

Table 5: (Determination of Nifuroxazide in laboratory prepared mixtures by applying the proposed methods 2D, 2DD).