Sterilization period

Carrier materials

Result

50 min

PVDF from Millipore

All positive in a total of 10 samples

 

PS from Millipore

All negative in a total of 10 samples

 

PS from Nihon Pall

All negative in a total of 10 samples

 

PS from Sartorius

All negative in a total of 10 samples

20 min

PS from Sartorius

All positive in a total of 10 samples

 

PS from Nihon Pall

Two negative samples in 10 samples

 

PC from Millipore

Six negative samples in 10 samples

 

PS from Millipore

All negative in a total of 10 samples

Sterilization condition:ozone gas concentration 3000ppm, relative humidity (RH) 80%, temperature 35°C, microorganism G. stearothermophilus ATCC 7953, 2.4 x 10³ CFU/sheet PVDF: polyvinylidene difluoride, PS: polysulfone, PC: polycarbonate

Table 1: Results from sterilization obtained by use of a biological indicator (BI).