Bacteria

Population (CFU)

G. stearothermophilus

Initial

25

25

25

 

Autoclave sterilization

28

21

27

 

Ozone gas sterilization

11

11

17

M. luteus

Initial

34

34

34

 

Autoclave sterilization

31

27

27

 

Ozone gas sterilization

0

0

0


Autoclaving was conducted at 121.1°C for 15 min. The concentration of ozone gas was 3000 ppm for 15 min. RH 80 %. PS is polysulfone.

Table 2: Results from cultivation of inoculated G. stearothermophilus ATCC 7953 or M. luteus onto PS from Millipore sterilized by autoclaving or by use of ozone gas