Test Article Fc Mol Wt EC50 in UT-7EPO Assay Terminal t1/2 (hr) Cl (mL/day/kg) Epoetin-a* NA 34 kDa 9.55 x 10-12 2.5 425 Darbepoetin-a NA 37 kDa 6.31 x10-12 6.9 115 CNTO 528 IgG1 62 kDa 2.08 x 10-10 37.0 56.81 NEM-2824 IgG1ala-ala 58 kDa 8.00 x 10-11 50.4 23.08 NEM-2825 IgG1ala-ala 58 kDa 7.69 x 10-11 48.0 20.32 NEM-2466 IgG4ala-alaS>P 58 kDa 9.36 x 10-11 53.3 30.01 NEM-2467 IgG4ala-alaS>P 58 kDa 1.01 x 10-10 45.8 28.93 CNTO 530 IgG4ala-alaS>P 58 kDa 6.60 x 10-11 72.7 19.00 CNTO 531 IgG4ala-alaS>P 58 kDa 8.27 x 10-11 71.5 16.78 *Pharmacokinetic values for a single IV dose of epoetin-α and darbepoetin-αin rats are taken from Egrie et al. [46].

Table 1: Characteristics of EPO-R Agonists used in this study.