Time (min) Cogent EE Mobile Phase A1 (± %modifier) Mobile Phase B2 (± %modifier) 0 1 99 5 1 99 30 60 40 40 60 40 11:1 Ethanol/IPA with 0.1% trifluoroacetic acid or 0.1% isopropyl Amine or mix of both was used with this study. 2Cyclohexane with 0.1% trifluoroacetic acid or 0.1% isopropyl amine or mix of both was used with this study.

Table 1: Representative Cellulose Carbamate Gradient Profile.