Time
Flow (mL/min)
%A
%B
%C
%D
0.0
1
99.5
0.5
0
0
2
2
90
5
0.5
4.5
5
2
60
19
2
19
(A) Hexane, (B) Ethanol, (C) Acetonitrile and (D) Isopropanol
Table 3:
Gradient conditions for Figure 1.