Time Flow (mL/min) %A %B %C %D
0.0 1 99.5 0.5 0 0
2 2 90 5 0.5 4.5
5 2 60 19 2 19

(A) Hexane, (B) Ethanol, (C) Acetonitrile and (D) Isopropanol
Table 3: Gradient conditions for Figure 1.