| Items |
Derivative method (PAR) |
DD1 method (DPH) |
Multivariate method |
Densitometric method |
Official method [4]* |
| PAR |
DPH |
| PLS |
PCR |
PLS |
PCR |
PAR |
DPH |
PAR |
DPH |
| Mean |
99.95 |
99.7 |
101.00 |
100.61 |
100.09 |
100.40 |
100.00 |
100.35 |
100.11 |
99.96 |
| SD |
1.33 |
0.99 |
1.20 |
1.42 |
0.75 |
1.10 |
0.93 |
0.86 |
1.14 |
1.02 |
| RSD % |
1.33 |
0.99 |
1.18 |
1.41 |
0.74 |
1.09 |
0.93 |
0.85 |
1.13 |
1.02 |
| n |
6 |
7 |
8 |
8 |
8 |
8 |
7 |
8 |
6 |
7 |
| Variance |
1.76 |
0.98 |
1.44 |
2.01 |
0.56 |
1.21 |
0.86 |
0.73 |
1.29 |
1.04 |
| Student's t-test |
0.071
(2.22) |
0.798
(2.17) |
0.943
(2.17) |
0.684
(2.17) |
0.734
(2.16) |
0.108
(2.16) |
0.251
(2.20) |
0.218
(2.16) |
- |
- |
| F-value |
1.365
(5.05) |
1.059
(4.28) |
1.107
(4.87) |
1.550
(4.87) |
1.857
(3.86) |
1.928
(4.20) |
1.489
(4.38) |
1.424
(3.86) |
- |
- |
* HPLC method using C18 column , phosphate buffer- acetonitrile (94 :6 by volume) as a mobile phase with UV detection at 225 nm at 4.6 and 8.3 min for PAR and DPH
respectively at a flow rate of 1 ml min-1 |
