| Trial-1 |
Trial-2 |
Trial-3 |
| Time |
Buffer
% A |
ACN:MeOH
% B |
Time |
Buffer
% A |
ACN:MeOH
% B |
Time |
Buffer
% A |
ACN:MeOH
% B |
| 0.00 |
100 |
0 |
0.00 |
85 |
15 |
0.00 |
70 |
30 |
| 10.00 |
95 |
5 |
10.00 |
85 |
15 |
3.50 |
70 |
30 |
| 15.00 |
85 |
15 |
15.00 |
57 |
46 |
4.50 |
30 |
70 |
| 30.00 |
70 |
30 |
30.00 |
56 |
44 |
5.50 |
70 |
30 |
| 40.00 |
40 |
60 |
40.00 |
30 |
70 |
7.00 |
70 |
30 |
| 345.00 |
100 |
0 |
45.00 |
85 |
15 |
|
|
|
| 50.00 |
100 |
0 |
50.00 |
85 |
15 |
|
|
|
|
