Figure 1: (A) Recovery plot for different applied amounts (AA) of the CB.Hep-1 mAb (phosphate/citric acid buffer system). (B) SDS-PAGE to demonstrate retention of the CB.Hep-1 mAb into the PASFF. Line 1, CB.Hep-1 mAb released from the PASFF after elution step. Line 2, CB.Hep-1 mAb released from the Sepharose 4 FF with the purified CB.Hep-1 mAb. Line 3, CB.Hep-1 mAb (15 μg) (SDS-PAGE control). Line 4, CB.Hep-1 mAb (20 μg) (SDS-PAGE control).