Figure 2: Partial overlay of the elution profiles of representative purification cycles (1, 10, 30, 60 and 100) of the life time study. (Left) 150 mM PBS, pH 8.0/100 mM citric acid, pH 3.0 buffer system. (Right). 1.5M glycine-NaOH/3M NaCl; pH 9.0/200 mM glycine-HCl; pH 2.5.