Figure 4: Results of the SDS-PAGE analysis to measure the purity of the CB.Hep-1 mAb purified by the PASFF affinity chromatography in the lifetime study (purification cycles: (1, 10, 30, 60 and 100). (Left) 150 mM PBS; pH 8.0/100 mM citric acid; pH 3.0 buffer system. (Right) 1.5M glycine-NaOH/3M NaCl; pH 9.0/200 mM glycine-HCl; pH 2.5 buffer system.