Parameters A B
Adsorption capacity (mg/mL of matrix) 9.0 ± 1.0 15.0 ± 0.2
Adsorption capacity (%) 67.8 ± 2.7 98.7 ± 8.3
Elution capacity (mg/mL of matrix) 7.8 ± 0.8 14.5 ± 0.2
Elution capacity (%) 86.6 ± 0.3 96.6 ± 0.4
Recovery (%) 52.3 ± 5.7 96.0 ± 1.9
Purity measured by SDS-PAGE (%) 99.2 ± 1.0 99.6 ± 0.5
Purity measured by size-exclusion-HPLC (%) 96.5 ± 0.8 97.0 ± 2.82
Mouse DNA content (mAb pg/mg total protein) < 7.5 < 7.5
Specific activity of purified CB.Hep-1 mAb (%) 98.3 ± 22.1 97.9 ± 10.2
mAb concentration (mg/mL) 5.6 ± 0.7 3.3 ± 0.6
Protein A concentration (ng/mL) 16.4  ± 1.2 4.2  ± 1.7
Protein A content (ppm) 2.9 ± 1.7 1.2 ± 1.2
Buffer system cost ($/g of purified mAb) 1.0 4.4
Productivity (mg mAb/L of matrix per hour) 50.9 96.1
Productivity increase (%) 100.0 188.6
Table 1: Results of the preliminary experiment II to purify the CB.Hep-1 mAb using ascites as complex biological source and both buffer systems (n=3). Buffer System A: 150 mM PBS, pH 8.0/100 mM citric acid, pH 3.0. Buffer System B: 500 mM glycine-NaOH/3M NaCl, pH 9.0/200 mM glycine-HCl, pH 2.5.