| Parameters |
A |
B |
| Adsorption capacity (mg/mL of matrix) |
9.0 ± 1.0 |
15.0 ± 0.2 |
| Adsorption capacity (%) |
67.8 ± 2.7 |
98.7 ± 8.3 |
| Elution capacity (mg/mL of matrix) |
7.8 ± 0.8 |
14.5 ± 0.2 |
| Elution capacity (%) |
86.6 ± 0.3 |
96.6 ± 0.4 |
| Recovery (%) |
52.3 ± 5.7 |
96.0 ± 1.9 |
| Purity measured by SDS-PAGE (%) |
99.2 ± 1.0 |
99.6 ± 0.5 |
| Purity measured by size-exclusion-HPLC (%) |
96.5 ± 0.8 |
97.0 ± 2.82 |
| Mouse DNA content (mAb pg/mg total protein) |
< 7.5 |
< 7.5 |
| Specific activity of purified CB.Hep-1 mAb (%) |
98.3 ± 22.1 |
97.9 ± 10.2 |
| mAb concentration (mg/mL) |
5.6 ± 0.7 |
3.3 ± 0.6 |
| Protein A concentration (ng/mL) |
16.4 ± 1.2 |
4.2 ± 1.7 |
| Protein A content (ppm) |
2.9 ± 1.7 |
1.2 ± 1.2 |
| Buffer system cost ($/g of purified mAb) |
1.0 |
4.4 |
| Productivity (mg mAb/L of matrix per hour) |
50.9 |
96.1 |
| Productivity increase (%) |
100.0 |
188.6 |
