Robustness
parameter |
Coumarin |
o-coumaric acid |
Dihydrocoumarin |
Syringaldehyde |
| Tf |
RSD% |
Rs |
RSD% |
Tf |
RSD% |
Rs |
RSD% |
Tf |
RSD% |
Rs |
RSD% |
Tf |
RSD% |
Rs |
| Methanol |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 29% |
1.03 |
0.36 |
16.3 |
1.65 |
1.04 |
0.14 |
5.80 |
5.45 |
1.03 |
0.17 |
17.4 |
1.54 |
1.13 |
0.49 |
- |
| 30%* |
1.04 |
16.5 |
1.04 |
5.38 |
1.03 |
17.1 |
1.14 |
- |
| 31% |
1.04 |
16.9 |
1.04 |
5.23 |
1.03 |
16.9 |
1.14 |
- |
| Acetonitrile |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 4.9% |
1.04 |
0.72 |
16.7 |
1.20 |
1.05 |
0.24 |
5.64 |
4.96 |
1.04 |
0.80 |
17.1 |
0.93 |
1.05 |
1.87 |
- |
| 5%* |
1.05 |
16.4 |
1.05 |
5.31 |
1.04 |
16.8 |
1.08 |
- |
| 5.1% |
1.06 |
16.4 |
1.05 |
5.11 |
1.04 |
16.9 |
1.09 |
- |
| Formic Acid |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 0.9% |
1.04 |
0.20 |
16.4 |
0.32 |
1.04 |
0.40 |
5.56 |
2.24 |
1.03 |
0.45 |
16.8 |
0.33 |
1.04 |
1.73 |
- |
| 1%* |
1.04 |
16.4 |
1.04 |
5.35 |
1.04 |
16.9 |
1.08 |
- |
| 1.1% |
1.04 |
16.3 |
1.03 |
5.35 |
1.04 |
16.9 |
1.06 |
- |
| Temperature |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23°C |
1.04 |
0.72 |
13.7 |
0.83 |
1.04 |
0.33 |
4.68 |
9.52 |
1.04 |
0.33 |
14.0 |
2.68 |
1.13 |
0.59 |
- |
| 25°C* |
1.05 |
13.7 |
1.04 |
4.32 |
1.04 |
14.2 |
1.12 |
- |
| 27°C |
1.06 |
13.9 |
1.04 |
3.88 |
1.03 |
14.7 |
1.12 |
- |
| Flow Rate |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 0.98 mL min-1 |
1.13 |
0.45 |
13.5 |
0.19 |
1.10 |
0.51 |
4.25 |
0.47 |
1.16 |
0.25 |
13.5 |
0.19 |
1.16 |
0.25 |
- |
| 1.0 mL min-1 |
1.14 |
13.4 |
1.10 |
4.26 |
1.17 |
13.4 |
1.17 |
- |
| 1.02 mL min-1 |
1.15 |
13.5 |
1.11 |
4.29 |
1.17 |
13.5 |
1.17 |
- |
|
| Data: *Optimized conditions; Tf, tailing factor; Rs, resolution |
| Table 4: Data of tailing factor and resolution in different conditions for robustness study. |
