Robustness
parameter

Coumarin

 

o-coumaric acid

 

Dihydrocoumarin

 

Syringaldehyde

Tf

RSD%

Rs

RSD%

 

Tf

RSD%

Rs

RSD%

 

Tf

RSD%

Rs

RSD%

 

Tf

RSD%

Rs

Methanol

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

29%

1.03

0.36

16.3

1.65

 

1.04

0.14

5.80

5.45

 

1.03

0.17

17.4

1.54

 

1.13

0.49

-

30%*

1.04

16.5

 

1.04

5.38

 

1.03

17.1

 

1.14

-

31%

1.04

16.9

 

1.04

5.23

 

1.03

16.9

 

1.14

-

Acetonitrile

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

4.9%

1.04

0.72

16.7

1.20

 

1.05

0.24

5.64

4.96

 

1.04

0.80

17.1

0.93

 

1.05

1.87

-

 5%*

1.05

16.4

 

1.05

5.31

 

1.04

16.8

 

1.08

-

5.1%

1.06

16.4

 

1.05

5.11

 

1.04

16.9

 

1.09

-

Formic Acid

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

0.9%

1.04

0.20

16.4

0.32

 

1.04

0.40

5.56

2.24

 

1.03

0.45

16.8

0.33

 

1.04

1.73

-

1%*

1.04

16.4

 

1.04

5.35

 

1.04

16.9

 

1.08

-

1.1%

1.04

16.3

 

1.03

5.35

 

1.04

16.9

 

1.06

-

Temperature

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

23°C

1.04

0.72

13.7

0.83

 

1.04

0.33

4.68

9.52

 

1.04

0.33

14.0

2.68

 

1.13

0.59

-

25°C*

1.05

13.7

 

1.04

4.32

 

1.04

14.2

 

1.12

-

27°C

1.06

13.9

 

1.04

3.88

 

1.03

14.7

 

1.12

-

Flow Rate

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

0.98 mL min-1

1.13

0.45

13.5

0.19

 

1.10

0.51

4.25

0.47

 

1.16

0.25

13.5

0.19

 

1.16

0.25

-

1.0 mL min-1

1.14

13.4

 

1.10

4.26

 

1.17

13.4

 

1.17

-

1.02 mL min-1

1.15

13.5

 

1.11

4.29

 

1.17

13.5

 

1.17

-

Data: *Optimized conditions; Tf, tailing factor; Rs, resolution
Table 4: Data of tailing factor and resolution in different conditions for robustness study.