TYPE SAMPLE POLARITY MOLECULAR WEIGHT RANGE STATIONARY PHASE MOBILE PHASE
Adsorption non-polar to somewhat polar 100 - 104 silica or alumina non-polar to polar
Partition (reversed-phase) non-polar to somewhat polar 100 - 104 non-polar liquid adsorbed or chemically bonded to the packing material relatively polar
Partition (normal-phase) somewhat polar to highly polar 100 - 104 highly polar liquid adsorbed or chemically bonded to the packing material relatively non-polar
Ion Exchange highly polar to ionic 100 - 104 ion-exchange resins made of insoluble, high-molecular weight solids functionalized typically with sulfonic acid (cationic exchange) or amine (anionic exchange) groups aqueous buffers with added organic solvents to moderate solvent strength
Size-Exclusion non-polar to ionic 103 – 106 small, porous, silica or polymeric particles polar to non-polar
Table 2: Different type of HPLC which are used for Method development for Pharmaceutical industries