| Detector |
Analytes |
Solvent Requirement |
Comments |
| UV-vis |
Any with chromophores |
UV-grade, non-UV absorbing solvent |
Has a degree of selectivity and is useful for many HPLC Application |
| Fluorescence |
Fluorescent compound |
UV-grade, non-UV absorbing solvent |
Highly selective and sensitive. Often used to analyzed derivatized compounds |
| Refractive Index (Ri) |
Compound with different RI to mobile Phase |
Cannot run mobile phase gradient |
Virtually a universal detector but has limited sensitivity |
| Conductivity |
Charged or polar compound |
Mobile phase must be Conducting |
Excellent for ion Exchange method |
| Electrochemical |
Readily oxidized or reduced compounds Especially biological sample |
Mobile phase must be conducting |
Very selective & sensitive |
| Evaporative light Scattering (ELSD) |
Virtually all compound |
Must use volatile solvents and volatile buffers |
A universal detector which is highly sensitive. Not selective |
| Mass spectrometer (MS) |
Broad range of compound |
Must use volatile solvent & volatile buffers |
Highly sensitive & powerful 2nd dimension analytical tool |
