Parameter Tz Deg
Accuracy (mean ± SD) 100.16 ± 1.17 99.77 ± 1.52
Specificity 99.68 ± 1.36 100.86 ± 1.09
Precision    
Repeatability * 99.59 ± 0.46 100.62 ± 0.55
Intermediate precision** 99.84 ± 0.55 100.78 ± 0.95
Linearity    
Wavelengths 268 and 308 nm 306 and 265 nm
Slope 0.0833 0.1186
Intercept -0.0089 0.0094
Correlation coefficient (r) 0.9998 0.9998
Range 1 – 10 μg/ml 1 – 10 μg/ml
Table 2: Assay validation sheet of the proposed methods for the determination of Tz and Deg.