Parameter

Tz

Deg

Accuracy (mean ± SD)

100.16 ± 1.17

99.77 ± 1.52

Specificity

99.68 ± 1.36

100.86 ± 1.09

Precision

 

 

Repeatability *

99.59 ± 0.46

100.62 ± 0.55

Intermediate precision**

99.84 ± 0.55

100.78 ± 0.95

Linearity

 

 

Wavelengths

268 and 308 nm

306 and 265 nm

Slope

0.0833

0.1186

Intercept

-0.0089

0.0094

Correlation coefficient (r)

0.9998

0.9998

Range

1 – 10 μg/ml

1 – 10 μg/ml

Table 2: Assay validation sheet of the proposed methods for the determination of Tz and Deg.