Product Proposed Method Standard addition Taken  μg/ml Added μg/ml Found  μg/ml Recovery   % Tz in Acnitaz® gel 0.1g% Tz 101.74 ± 1.22 4.00 2.00 2.04 102.00 4.00 3.90 97.50 6.00 6.08 101.33 Mean 100.28 SD 2.43 RSD% 2.42

Table 4: Determination of Tz and Deg in Acnitaz® gel by the proposed method and application of standard addition technique.