Figure 2: Effect of Fetal Bovine Serum (FBS) and additive Platelet-derived Growth Factor (PDGF)-BB on contraction of collagen gels containing Porcine Trabecular Meshwork Cells (pTMCs). (A) Representative photographs of a collagen gel stained by crystal violet after 48 h culture. (B) Effect of PDGF-BB on both concentration of FBS-stimulated collagen gel contraction and on cell viability. Various concentrations of PDGF-BB solution were added and the cells were cultured on collagen gels in the presence of FBS at a concentration of 0.5% or 2%. The areas of collagen gels were measured and data were shown as % of collagen gel area without FBS. Open columns are data for 0.5% FBSstimulated contraction and solid columns are data for 2% FBS. Symbols, - or + indicated absence or presence of FBS. Dotted line indicates cell viability (%). Data represent the mean SE of triplicate measurements. *P<0.05, ***P<0.005 versus 0.5% FBS alone, #P<0.05, ###P<0.005 versus 2% FBS alone.