Figure 4: Effects of ethacrynic acid (A), thapsigargin (B), and simvastatin (C) on collagen gel contraction induced by 0.5% or 2% Fetal Bovine Serum (FBS) and on cell viability. Various concentrations of inhibitors were added and cells were cultured on collagen gels in the presence of FBS at a concentration of 0.5% or 2%. The areas of collagen gels were measured and data are shown as % of collagen gel area without FBS. Open bars indicate 0.5% FBS-stimulated contraction and solid bars indicate 2% FBS. Symbols, “-” or “+” indicate absence or presence of FBS. Dotted line indicates cell viability (%). Data represent the mean ± SE of triplicate measurements..*P<0.05, ***P<0.005 versus 0.5% FBS alone, ##P<0.01, ###P<0.001 versus 2% FBS alone.