Parameters

Cell
Lines

T-flask (SCM)

T-flask (PFM)

R-bottle
(SCM)

R-bottle
(PFM)

GPM
(SCM)

GPB
(PFM)

HFB
(SCM)

HFB
(PFM)

Time of experiment (days)

A

9

9

10

10

20

28

32

37

B

9

9

10

10

21

28

32

32

C

8

8

10

9

28

28

33

28

Maximum EGR (h-1)

A

0.032

0.024

0.024

0.034

0.044

-

-

-

B

0.037

0.037

0.037

0.058

0.032

0.026

-

-

C

0.032

0.068

0.035

0.026

0.032

0.043

-

-

Maximum cell density (cells/mLx106)

A

2.38±0.125

1.37±0.1000

2.21±0.085

1.66±0.127

10.2

0.63

-

-

B

2.10±0.066

1.46±0.075

2.95±0.135

1.50±0.127

10.5

20.5

-

-

C

1.93±0.185

1.49±0.091

2.69±0.271

1.79±0.096

11.7

16.7

-

-

Time to reach the maximum cell density (days)

A

6

6

8

9

7

26

-

-

B

7

7

8

9

14

14

-

-

C

7

7

9

7

19

14

-

-
Table 1: Results of the cell culture behaviour of CB.Hep-1 (A), CB.Ifn-2.4 (B) and ETH.13-15 (C) hybridomas using SCM and PFM in different vessels. The number of experiments were for T-flask (n=3), roller-bottle (n=3), GPM (n=1, except for hybridoma CB.Hep-1 in PFN, (n=2)) and HFB (n=1). Values of maximum cell density represent the mean and confidence interval (a = 95%).