Parameters

Cell Lines

T-flask (SCM)

T-flask (PFM)

R-bottle (SCM)

R-bottle (PFM)

GPB
(SCM)

GPB
(PFM)

HFB (SCM)

HFB (PFM)

Maximum IgG conc.
(μg/mL)

A

15.4±3.571

18.7±3.762

20.3±6.027

13.1±0.753

1134.1

475.0

1349.0

1455.2

B

21.1±3.282

18.4±5.032

37.7±4.901

13.7±0.907

1466.0

1944.8

1540.0

1944.9

C

52.5±8.094

29.1±5.093

91.8±9.956

48.1±7.650

4700.1

5785.0

4456.3

3250.1

Time to reach
maximum IgG conc.
(days)

A

7

7

8

8

9

18

25

31

B

7

7

7

9

11

22

21

22

C

9

8

9

8

23

26

21

26

Maximum IgG prod.
(pg/cell)

A

13.3±0.643

16.2±3.394

29.5±4.756

16.5±4.170

110.0

754.0

-

-

B

21.2±2.781

13.2±3.470

17.7±5.102

11.8±2.777

160.8

113.5

-

-

C

31.4±6.820

20.8±6.552

40.6±5.151

32.9±4.530

487.6

364.2

-

-

Average IgG prod.
(mg/day)

A

0.2

0.3

1.5

1.3

9.3

1.9

2.5

2.0

B

0.3

0.2

2.6

1.3

6.7

6.4

3.7

3.5

C

0.7

0.8

1.4

1.8

8.5

12.1

8.8

4.1

Total IgG prod.
(mg IgG/run)

A

2.3

2.3

20.0

13.1

186.6

51.5

80.6

72.2

B

2.6

2.1

35.1

13.4

139.7

181.9

117.8

114.9

C

7.6

4.1

77.9

53.1

239.4

341.0

291.1

155.6

Table 2: Results of the mAb production by CB.Hep-1 (A), CB.Ifn-2.4 (B) and ETH.13-15 (C) hybridomas in SCM and PFM. The number of experiments were: T-flask (n=3), roller-bottle (n=3), GPM (n=1, except for CB.Hep-1 hybridoma in PFN, (n=2)) and HFB (n=1). Values of maximum IgG concentration and maximum IgG production represent the mean and confidence interval (a = 95%).