Sr .No. |
Parameter |
Level |
RT*
(min) |
As USP** |
Res USP*** |
1 |
Flow rate (mL/min) |
0.9 |
7.18 |
1.21 |
2.16 |
1.0 |
6.41 |
1.18 |
2.18 |
1.1 |
5.92 |
1.16 |
2.06 |
2 |
pH of the buffer |
3.0 |
6.41 |
0.97 |
2.23 |
3.3 |
6.5 |
1.16 |
2.15 |
3 |
Column temperature (oC) |
25 |
6.41 |
1.18 |
2.18 |
31 |
6.12 |
1.17 |
2.12 |
37 |
6.1 |
1.2 |
2.02 |
4 |
Composition of solvent B (%) |
49 |
5.97 |
1.2 |
2.01 |
50 |
6.41 |
1.18 |
2.18 |
51 |
6.3 |
1.17 |
2.08 |
5 |
Injection volume (µL) |
20 |
6.41 |
1.18 |
2.18 |
50 |
6.41 |
1.14 |
1.62 |
6 |
Wavelength λ max-(nm) |
275 |
6.44 |
1.22 |
2.1 |
280 |
6.41 |
1.18 |
2.18 |
285 |
6.48 |
1.18 |
2.14 |
*RT- Retention Time, **As USP- Asymmetry USP, *** Res USP- Resolution USP |